Journal: Cancers

Article Title: Multiparametric Characterization of the DSL-6A/C1 Pancreatic Cancer Model in Rats

doi: 10.3390/cancers16081535

Figure Lengend Snippet: List of antibodies used for the immunological analysis of the DSL-6A/C1 tumors.

Article Snippet: CD8a , PerCP-Vio 700 , REA437 , Human cell line , Miltenyi , 130-132-092.

Techniques:

Journal: Cancers

Article Title: Multiparametric Characterization of the DSL-6A/C1 Pancreatic Cancer Model in Rats

doi: 10.3390/cancers16081535

Figure Lengend Snippet: Representative flow cytometry plots and detailed gating strategy of tumor-infiltrating lymphocytes in DSL-6A/C1 tumors. Dead cells were excluded using a zombie dye (alive). Lymphocytes were selected by gating for size and granularity (Forward Scatter Area (FSC-A) versus Side Scatter Area (SSC-A)). Living lymphocytes were further plotted by SSC-H versus SSC-A to gate single cells. From the single cells (singles), lymphocytes (CD45 + ) were gated based on their CD45 expression. B cells were defined as CD45 + lymphocytes and the expression of CD45RA (B cells). T cells were defined by gating on CD45 + lymphocytes and the expression of CD3 (T cells), whereas CD3 cells expressing CD161 were considered NK cells (NK cells). T cells were further divided into CD8 T cells (CD8 T cells) or CD4 T cells (CD4 T cells).

Article Snippet: CD8a , PerCP-Vio 700 , REA437 , Human cell line , Miltenyi , 130-132-092.

Techniques: Flow Cytometry, Expressing

Journal: bioRxiv

Article Title: Parotid glands have a dysregulated immune response following radiation therapy

doi: 10.1101/2023.11.27.568872

Figure Lengend Snippet: Parotid glands were prepared as described in . A) Viable CD3+ T cells. B) Viable CD3-cells. C) Viable CD49b+ NK cells. D) Viable CD4+ T cells. E) Viable CD8+ T cells. F) Viable CD4+CD8+ double positive T cells. G) Viable CD4-CD8-double negative T cells. Data analyzed by one-way ANOVA with Tukey’s post-hoc test and represented as mean ± SEM. Treatment groups with the same letter are not statistically different from each other.

Article Snippet: An eight-color direct immunofluorescence protocol was used to label digested murine salivary glands with the following monoclonal antibodies: VioBlue-labeled Ly-6G, VioGreen-labeled CD11b, APC-labeled F4/80, APC-Vio 770-labeled CD45, VioBlue-labeled CD4, VioGreen-labeled CD8a, FITC-labeled CD3, and PE-Vio 770-labeled CD49b (Miltenyi Biotec, Auburn, CA) and analyzed by flow cytometry (Miltenyi Biotec MacsQuant, Auburn, CA).

Techniques:

Journal: bioRxiv

Article Title: Parotid glands have a dysregulated immune response following radiation therapy

doi: 10.1101/2023.11.27.568872

Figure Lengend Snippet: For analysis at day 5, IGF-1 was injected via tail vein at day 4 post IR, and parotid tissue was harvested 24 hours later. For analysis at day 30, IGF-1 was injected via tail vein at days 4, 5, and 6 following IR, and parotid tissue was dissected at day 30 post IR. A) Viable CD3+ T cells. B) Viable CD3-cells C) Viable CD49b+ NK cells. D) Viable CD4+ T cells. E) Viable CD8+ T cells. F) Viable CD4+CD8+ double positive T cells. G) Viable CD4-CD8-double negative T cells. Data analyzed by one-way ANOVA with Tukey’s post-hoc test and represented as mean ± SEM. Treatment groups with the same letter are not statistically different from each other.

Article Snippet: An eight-color direct immunofluorescence protocol was used to label digested murine salivary glands with the following monoclonal antibodies: VioBlue-labeled Ly-6G, VioGreen-labeled CD11b, APC-labeled F4/80, APC-Vio 770-labeled CD45, VioBlue-labeled CD4, VioGreen-labeled CD8a, FITC-labeled CD3, and PE-Vio 770-labeled CD49b (Miltenyi Biotec, Auburn, CA) and analyzed by flow cytometry (Miltenyi Biotec MacsQuant, Auburn, CA).

Techniques: Injection